Effect of ATP treatment on mRNA levels of NFAT target genes. PC12-NFAT-Luc cells were treated with varying concentrations of ATP as indicated and FK506 (10 nM) for 3 hours. RCAN1-4 mRNA and exon VI-containing BDNF mRNA were measured by real-time PCR quantification, and the results are shown as the fold induction relative to untreated cells. The graph shows the means ± SD of 3 independent experiments. Statistical significance is indicated for differences of ATP-treated cells vs. the control cells and for FK506 + 300 μM ATP-treated cells vs. cells treated only with 300 μM ATP (*, p < 0.05; **, p < 0.01. ***, p < 0.001).
Prasai et al. BMC Neuroscience 2011 12:90 doi:10.1186/1471-2202-12-90