Figure 6.

ATP-mediated sustentacular cell CREB phosphoryation is dependent on adenylyl cyclase III. (A) Olfactory epithelia were incubated with ATP to activate purinergic receptors, resulting in CREB phosphorylation in sustentacular cells, OSNs, and immature OSNs not expressing OMP. Using an inhibitor to ACIII blocked the phosphorylation of CREB in OSNs, immature OSNs not expressing OMP, and in sustentacular cells to different extents. (B) Fluorescence intensities were quantified as before. Bar chart shows mean ± SD. Sustentacular cells 2 h ATP (7.52 ± 0.47 n = 78), +SQ22536 (4.39 ± 0.24 n = 78), OSNs 2 h ATP (1.81 ± 2.27 n = 75), +SQ22536 (0.84 ± 0.19 n = 69, p = 0.18), immature cells 2 h ATP (3.05 ± 0.91 n = 79), +SQ22536 (1.01 ± 0.27 n = 80), p*** < 0.001. Scale bar 10 μm.

Dooley et al. BMC Neuroscience 2011 12:86   doi:10.1186/1471-2202-12-86
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