Mapping the methylation status of the PCDHB1 (A) and PCDH7 (B) regions in SH-SY5Y cells by bisulfite genomic DNA sequencing. The top diagram depicts the 5' flanking region of each gene with its transcription start site. CpG island regions are shown as gray areas. Methylated-CpG sites are shown as closed circles and unmethylated CpG sites as open circles. The triangles represent the CpG sites with A/T bases ([A/T]>4) located 1-3 or 6-9 base pairs from the CpG sites, indicating the putative MeCP2-binding sequences. Arrows at the bottom indicate the region that was amplified for ChIP-PCR. (A) 5' flanking region of the PCDHB1 gene. (B) 5' flanking region of the PCDH7 gene.
Miyake et al. BMC Neuroscience 2011 12:81 doi:10.1186/1471-2202-12-81