Western blots of p-GSK-3β, p-p38, p-ERK, and p-JNK levels in brain stem, hippocampus, frontal cortex, and cerebellum of PDGF-α-Syn overexpressing transgenic mice. Brain stem [BS], hippocampus [HP], frontal cortex [FC], and cerebellum [CB] from PDGF-α-Syn transgenic mice and litter-mate non-transgenic mice [WT] were solubilized in RIPA buffer and analyzed by Western blots for p-GSK-3β [A], p-p38 [B], p-ERK [C], and p-JNK [D]. p-GSK-3β was normalized to GSK-3β, p-p38 to p38, p-ERK to ERK, and p-JNK to JNK. p-p38MAPK was not detected in brain stem and therefore this data is not shown. All values are expressed as percent change relative to changes observed in WT control animals. Results are from 3-4 animals per group; [*, p < 0.05] and [**, p < 0.01] compared to corresponding protein levels in WT animals. All blots are representative of samples.
Kaul et al. BMC Neuroscience 2011 12:79 doi:10.1186/1471-2202-12-79