Additional File 3.

Comparison between larva processed for WISH with antisense and sense probes reveals low background staining. Embryos were raised in a 14:10 h L/D cycle and fixed and processed for WISH using aanat2 antisense or sense probe. Note that the sense probe produces no detectable signal, as it would recognize antisense mRNA, which should not be present. The brown regions are melanocytes in the skin, which have a natural pigment. All images are dorsal views, anterior to the top, with the pineal indicated (closed arrowheads). Position within the photoperiod is indicated by ZT and light conditions by the white (light period) and black (dark period) bars. Representative images are shown. Scale bar = 30 μm.

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Noche et al. BMC Neuroscience 2011 12:7   doi:10.1186/1471-2202-12-7