Open Access Highly Accessed Research article

Mutation of a nicotinic acetylcholine receptor β subunit is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae

Chris Bass1*, Alin M Puinean1, Melanie Andrews2, Penny Cutler3, Miriam Daniels3, Jan Elias2, Verity Laura Paul2, Andrew J Crossthwaite3, Ian Denholm1, Linda M Field1, Stephen P Foster1, Rob Lind3, Martin S Williamson1 and Russell Slater2

Author Affiliations

1 Centre for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, AL5 2JQ, UK

2 Syngenta Crop Protection, Werk Stein, Schaffhauserstrasse, Stein, CH4332, Switzerland

3 Syngenta Crop Protection, Jealotts Hill Intl. Research Centre, Bracknell, RG42 6EY, UK

For all author emails, please log on.

BMC Neuroscience 2011, 12:51  doi:10.1186/1471-2202-12-51

Published: 31 May 2011

Additional files

Additional file 1:

Selected-Reaction-Monitoring transitions for selected imidacloprid metabolites. Molecular weight, SRM transitions, collision energies and availability of authentic standards are detailed for 11 imidacloprid metabolites.

Format: DOC Size: 35KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

ESTs identified by microarray analysis significantly differentially transcribed between the insecticide resistant M. persicae clone FRC and the susceptible clone 4106A. The full list of ESTs along with Log2, calculated fold-change values and a description based on the closest BLAST hit is detailed.

Format: XLS Size: 134KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Fold change in expression of CYP6CY3 and additional P450 genes in the M. persicae clones FRC and 5191A (compared to the susceptible reference clone 4106A) as determined by quantitative PCR. For each EST the most similar Acyrthosiphon pisum P450 gene is detailed.

Format: DOC Size: 34KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 4:

Sequences of primers used in this study. Primer sequences are listed along with the purpose for which they were used.

Format: DOC Size: 56KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data