Photomicrographs showing the expression of Lgi1. In A E9.5 embryos showing a coronal view of the cephalic region with high-level Lgi1 expression in the primitive eye (pe). In B, a sagittal view of the E10.5 mouse whole embryo (anti-GFP-peroxidase stained) indicating regions seen in Figures B.1-5 showing the dorsoventral patterning of Lgi1 expression in the brain. The highest levels of Lgi1 expression were seen (B.3) in the roof of the hindbrain (rh), (B.4) mesenchymal connective tissue (cm) and (B5) dorsal root ganglion (drg). In C, a frontal section (H & E staining) of an E10.5 mouse brain identifying regions shown in Figure (C.1), identifying Lgi1 expression in the otic vesicle (ov) and facio-acoustic ganglion (fag). Other abbreviations: Skin ectoderm (se), neuroepithelium (ne), neural tube (ne), telencephalic vesicle (tv), wall of telencephalic vesicle (wtv), olfactory placode (op), lamina terminalis (lt), peripheral margin of the Rathke's Pouch (pmrp), mandibular component of the first brachial arch (mc), 4th ventricle (4thv), neural lumen (nl). Scale bars = 100 μm (B.4, C.1), 50 μm (A, B.1, B.2), 10 μm (B.5), 5 μm (B.3).
Silva et al. BMC Neuroscience 2011 12:43 doi:10.1186/1471-2202-12-43