Additional file 6.
Time-lapse imaging of the cranial slice in Figure 3C showing only nuclear H2B-RFP labeled ectoderm and ectoderm-derived cells to follow movements of placodal cell bodies within the ectoderm and during delamination. Two examples of placodal cells that move from within the ectoderm are each marked by a different colored dot (red and green) starting at a time point when the cell can initially be tracked. Tracking of the green cell begins after it has already begun migration. Some cells move in different directions before appearing to follow a path to the site of exit on the basal side of the epithelium (see red cell). 210 minutes in real time taken at 3-minute intervals; 45 um z-stack; 20×; 10 fps.
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Shiau et al. BMC Neuroscience 2011 12:37 doi:10.1186/1471-2202-12-37