Additional file 4.


Time-lapse imaging of cell divisions (dotted lines around cells) in the trigeminal placodal ectoderm as labeled by nuclear H2B-RFP and membrane GFP. Dotted white line demarcate basal edge of ectoderm. Yellow arrow points to a placodal cell that divides prior to ingression and a different placodal cell (blue arrow) undergoes division after it is already in the mesenchyme. Video covers 336 minutes of development in real time captured at 3-minute intervals; 45 um z-stack; 20×; 7.5 fps.

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Shiau et al. BMC Neuroscience 2011 12:37   doi:10.1186/1471-2202-12-37