Additional file 1.


Time-lapse movie of a representative cranial slice in bright-field. The image sequence is taken from the middle slice of a z-stack over the duration of the time-lapse imaging (8.4 hours). For each z-stack, a bright-field image was taken at the middle slice as control to monitor integrity of tissue in parallel with the fluorescence imaging. Images were taken at 3-minute intervals at 20×, and shown at 6.5 fps. ecto, ectoderm; mes, mesenchyme; nt, neural tube.

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Shiau et al. BMC Neuroscience 2011 12:37   doi:10.1186/1471-2202-12-37