CHAPS-insoluble membrane fractions in Fa2h-/-/Cgt-/- myelin. Myelin from wild-type (Fa2h+/+/Cgt+/+) (A), Fa2h+/-/Cgt-/- (B), and Fa2h-/-/Cgt-/- (C) mice isolated by sucrose density gradient centrifugation was treated with 20 mM CHAPS at 37°C for 30 min, followed by Optiprep density gradient centrifugation. Gradient fractions were collected from the top, and total lipids were extracted from all gradient fractions and separated by TLC. Most membrane lipids were present in the CHAPS-insoluble membrane fractions (CIMF), independent of the mouse genotype. Positions of lipid standards are indicated. These experiments have been done two times using two independent myelin preparations with similar results. PE, phosphatidylethanolamine; SM, sphingomyelin.
Meixner et al. BMC Neuroscience 2011 12:22 doi:10.1186/1471-2202-12-22