Expression of transcripts encoding different Kalirin isoforms varies in nucleus accumbens and prefrontal cortex. A. The proteins encoded by the major isoforms of rat and human Kalirin are drawn to scale; although Δ-isoforms of Kal8, -9 and -12 have been identified [26-28], only ΔKal7 is depicted. The ΔKal isoforms are produced when the ΔKal promoter is used instead of the A, B, C, or D promoters diagramed in Figure 2. The sense and anti-sense primers used for real-time Q-PCR are shown in red; 5'- and 3'-untranslated regions of the corresponding transcripts are indicated by red lines. The Kal7-specific and Kal-spectrin antibody epitopes are indicated by blue lines. B. RNA prepared from mouse nucleus accumbens (NAc) and prefrontal cortex (PFC) was subjected to real-time Q-PCR analysis using the ΔKal and full-length primers; three separate preparations of RNA from each tissue were analyzed in triplicate and data were normalized to GAPDH. C. PFC and NAc expression of Kalirin isoforms was examined via Q-PCR analysis of 3'-terminal exons encoding Kal7, Kal8, Kal9 and Kal12. Higher levels of Kal7 transcript were seen in the PFC than the NAc.
Mains et al. BMC Neuroscience 2011 12:20 doi:10.1186/1471-2202-12-20