Full-length Kalirin promoter usage varies in different brain regions. A. Putative promoter regions (red line) and initiation exons in the mouse Kalrn gene, which is located on mouse chromosome 16, were identified based on homology to human and rat Kalrn [27,28]; translational start sites are indicated by bent arrows. The introns separating promoters B, C, A and D, which produce full-length Kalirin, are not drawn to scale. The location of each initiation exon is indicated by the nucleotide number below the line; the location of the initiation exon-specific forward primers is indicated, along with the common reverse primer in Exon 3. B. RNA prepared from adult mouse prefrontal cortex (PFC), hippocampus (Hippo) and nucleus accumbens (NAc) was subjected to Q-PCR analysis using these primers; data were normalized to GAPDH. The same data are plotted on a log scale to allow visualization of data for full-length Kalirin promoters A and D, and as a percentage of the total in each brain region.
Mains et al. BMC Neuroscience 2011 12:20 doi:10.1186/1471-2202-12-20