Table 2

Primers used in cloning, chromosomal mapping and real-time RT-PCR for porcine Dab1 and Fyn isoforms

Primers

Application

Size(bp)

Sequence (5' to 3')


P1

Porcine Dab1 cloning

1867/1750

5'-ATGTCAACTGAGACAGAACTTCAAGTAGC-3'

P2

5'-TCCCTCTTCCAAGCGAGTTCC-3'

P3

Porcine Fyn cloning

1614/1605

5'-ATGGGCTGTGTGCAATGTAAGGA-3'

P4

5'-TTACAGGTTTTCACCGGGTTGATAC-3'

P5

sDab1 mapping

321

5'-ATGACCGACTGGGCTTACTGTTC-3'

P6

5'-CCAAATCACACAGCTTGAAAGAGTC-3'

P7

sFyn mapping

261

5'-CTGCCTCAGATTTCCAGACGC-3'

P8

5'-AGCAGGATGAGGTCAGGAGCC-3'

P9

sDab1 expression

207

5'-CCATCCAAATCATCTGCATCCC-3'

P10

5'-GACCTGTGCTATCTAGCTACCGGC-3'

P11

sDab1-Li expression

176

5'-GTATCAGGTTCCCACCAGCCAA-3'

P12

5'-GGGTCACAGCCTTTGTTGCCT-3'

P13

sFyn-B expression

179

5'-AAAGGGATGCCAAGGCTTACC-3'

P14

5'-GACATTGTGCCTGGTTTGAGAGTC-3'

P15

sFyn-T expression

186

5'-AACTGTGATTGCATCGAGTTGTACC-3'

P14

5'-GACATTGTGCCTGGTTTGAGAGTC-3'

P17

GAPDH expression

220

5'-GCAAATTCCACGGCACAGTCA-3'

P18

5'-TCAGCAGAAGGGGCAGAGATG-3'


Long et al. BMC Neuroscience 2011 12:17   doi:10.1186/1471-2202-12-17

Open Data