Table 1

Electrophysiological properties of chemically identified neurons in the rat medial septal/diagonal band complex

nestin+

nestin- & ChAT+

GAD67+

VGLUT+


number of neurons

33

32

13

9

Cm (pf)

55.76 ± 3.05 b

49.60 ± 2.37 b

76.99 ± 9.60

43.58 ± 3.92 b

Rm (MΩ)

400.56 ± 26.86

387.26 ± 27.27

289.63 ± 44.25

570.07 ± 120.25

Tau (ms)

0.85 ± 0.07

0.70 ± 0.05a

1.27 ± 0.18

0.64 ± 0.06 a

RMP (mV)

-56.73 ± 1.05

-59.75 ± 1.14

-55.62 ± 1.91

-58.00 ± 3.47

AHP duration (ms)

223.77 ± 12.08 b

230.52 ± 8.48 b

165.23 ± 7.04

238.17 ± 16.97 a

AHP amplitude (mV)

4.49 ± 0.21 b

5.02 ± 0.35 b

3.27 ± 0.25

4.80 ± 0.28 b

AP amplitude (mV)

99.04 ± 1.37 a

97.39 ± 1.22 b

104.97 ± 2.22

98.85 ± 2.82

Spike half width (ms)

0.88 ± 0.04 b

0.94 ± 0.03 b

0.67 ± 0.06

0.99 ± 0.05 b

Spike width (ms)

2.46 ± 0.09 b

2.66 ± 0.06 b

2.02 ± 0.14

2.78 ± 0.14 b

rise time (ms)

0.30 ± 0.01 b

0.32 ± 0.01 b

0.23 ± 0.02

0.32 ± 0.01 b

rise slope (mV/ms)

121.91 ± 8.30

105.85 ± 4.99 a

196.45 ± 24.41

106.31 ± 8.06 a

decay time (ms)

0.69 ± 0.03 b

0.73 ± 0.03b

0.51 ± 0.05

0.82 ± 0.06 b

decay slope (mV/ms)

-49.57 ± 3.21 a

-43.69 ± 1.81 b

-83.06 ± 9.41

-38.98 ± 2.55 b

MF (Hz)

8.39 ± 0.45 b

8.19 ± 0.49 b

18.38 ± 1.73

9.22 ± 0.97 b

FMAX (Hz)

20.73 ± 2.07 a

18.86 ± 1.77 b

28.99 ± 1.93

19.83 ± 0.82 b

FSTEADY (Hz)

6.86 ± 0.53 b

6.67 ± 0.35 b

17.23 ± 1.66

7.01 ± 0.49 b

spike adaptation

0.55 ± 0.05 b

0.56 ± 0.04 b

0.28 ± 0.06

0.56 ± 0.11 a

depolarizing sag (mV)

2.74 ± 0.62 b

1.81 ± 0.32 b

16.81 ± 0.77

2.22 ± 0.56 b

Ih (pA)

-12.90 ± 2.57 b

-4.00 ± 2.17 b

-186.82 ± 15.73

-3.49 ± 1.86 b, c


Nestin+, nestin mRNA-positive neuron (including nestin mRNA-positive & ChAT mRNA-negative neurons and nestin mRNA-positive & ChAT mRNA-positive neurons); Nestin- & ChAT+, nestin mRNA-negative and choline acetyl transferase mRNA-positive neuron; GAD67+, glutamic acid decarboxylases 67 mRNA-positive neuron; VGLUT+, vesicular glutamate transporter 1 or/and 2 mRNA-positive neuron. Cm, membrane capacitance; Rm, membrane resistance; Tau, time constant; RMP, resting membrane potential; threshold, action potential threshold; AHP, afterhyperpolarization; MF, Mean firing rate; FMAX, maximal firing frequency; FSTEADY, steady firing frequency. Ih, hyperpolarization activated current of neurons when hyperpolarized from -50 to -120 mV. The current pulses used for membrane property analyses were 0.1-0.3 nA. All values are presented as means ± S.M.E. Significant differences are identified: a versus GAD67+ neurons, P < 0.05; b versus GAD67+ neurons, P < 0.01; c versus nestin+ neurons, P < 0.05.

Zhu et al. BMC Neuroscience 2011 12:129   doi:10.1186/1471-2202-12-129

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