Aging alters expression of NLRP1 inflammasome components. Representative immunoblots and densitometric analysis of immunoblots for caspase-1 (Casp1), caspase-11 (Casp11), NLRP1, ASC, cleaved XIAP, P2X7 receptor (P2X7R) and pannexin-1 (PanX1) in brain lysates of young (Y) and aged (A) animals. Protein levels of cleaved caspase-1 and -11 are higher in aged animals compared to young. Protein levels of NLRP1 and ASC did not change with age whereas P2X7 receptor, the pannexin-1 protein and the cleaved fragment of XIAP are higher in aged animals than in young animals. β-Tubulin was used as an internal standard and control for protein loading. Data are presented as mean +/- SEM. *p < 0.05, **p < 0.01, ***p < 0.005 compared with young. n = 6 per group.
Mawhinney et al. BMC Neuroscience 2011 12:123 doi:10.1186/1471-2202-12-123