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Open Access Highly Accessed Research article

Calcitonin gene-related peptide (CGRP) and its receptor components in human and rat spinal trigeminal nucleus and spinal cord at C1-level

Sajedeh Eftekhari* and Lars Edvinsson

Author Affiliations

Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University, Lund, Sweden

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BMC Neuroscience 2011, 12:112  doi:10.1186/1471-2202-12-112

Published: 10 November 2011

Additional files

Additional file 1:

Movie: 3-dimensionally view of staining with CGRP (green) and IB4 (red) in rat C1. The two markers are most often not expressed in the same fibers. Occasionally the two markers co-localize in fibers (yellow).

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Additional file 2:

Movie: 3-dimensionally view of staining with CGRP (red) and RAMP1 (green) in rat C1. The neuropeptide CGRP and the receptor component RAMP1 are found within the same laminae, but they are not co-expressed in the same structures.

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Additional file 3:

Blocking peptide experiments. CLR and RAMP1 antibodies were pre-absorbed with their respective blocking peptides. No positive immunoreactivity is found when the blocking peptides are used. Asterisks point at autofluorescent lipofuscin in the human samples. Human STN (A) preabsorption for CLR, (B) preabsorption for RAMP1. Rat STN (C) preabsorption for CLR, (D) preabsorption for RAMP1. Human C1 (E) preabsorption for CLR, (F) preabsorption for RAMP1. Rat C1 (G) preabsorption for CLR, (H) preabsorption for RAMP1.

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