Anterograde BDA-Labelled Fibres in the Spinal Cord of Liposome-Treated Animals. A) Histology-processed section through the SCI epicentre (black asterisk) revealed extent of lesioning through the dorsal columns (dashed line; shown as insert in panel B). B) Montage of lesion site (black asterisk) demonstrating anterograde BDA-labelled fibres present only in the distal stump of the spinal cord (D), with no visible fibres found in the proximal stump (C). E-G) Closer observation distally revealed extensive neuronal collapse and retraction of BDA-labelled fibres evident by their bulb-like structure (black arrows). BDA = biotinylated dextran amine, DCC = dorsal column cut. Directional key: D = dorsal, V = ventral, C = caudal, R = rostral. Scale bars, A (horizontal bar) 500 μm and (vertical bar) 1 mm, montage B 800 μm, C-E 200 μm, F-G enlarged views 100 μm.
Aguilar Salegio et al. BMC Neuroscience 2011 12:11 doi:10.1186/1471-2202-12-11