Additional file 2.

KCl response of specific ORNs targeted by OR22a-GAL4 following a standard odor-induced adaptation protocol. Mean (+/- SEM) of the total amount of emitted photons of the Ca2+-induced response (within the ROI) evoked by the standard adaptation protocol (5 s of citronella, 5 successive applications, at 5-min intervals). KCl (70 mM) is applied 5 min after the last odor application. Since KCl depolarizes the neurons and induces a massive entry of calcium, it burns all remaining GA probe. We remark that KCl response goes up to about 18000 photons (about 18X higher than the fifth odor application), demonstrating that large amounts of GA probe were still available after the fifth odor application, implying that the gradual decrease of the response following the 5 successive odor applications is due to an adaptation process, and conversely, that GA is not a limiting factor.

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Murmu et al. BMC Neuroscience 2011 12:105   doi:10.1186/1471-2202-12-105