Table 4

Sequences of primers used for real-time PCR

Target Protein

Forward Primer

Reverse Primer

PCR Product Size (bp)

Sequence Accession No.

CACN

5'-TCACCATTGCCTCCGAACACTA-3'

5'-CAGGAGCATTTCTGCCGTGA-3'

104

NM012517

TrkA/B

5'-GCCACACAATGTTGCCCATC-3'

5'-AAGGACTCTGCCCTGGGTGA-3'

185

NM012731

FGFR

5'-TTGCCGAATGAAGACCACGA-3'

5'-GGAGTTCATGGACGAGCTGGA-3'

130

NM001109892

PDGFR

5'-GAATGACCACGGCGATGAGA-3'

5'-GGATAAGCCTCAAACACCACCTG-3'

141

NM031525

G12

5'-CAAGATGCTGGTGGCTGTCAA-3'

5'-AGCAGCTCTGCCTCACGATG-3'

81

NM021589

Raf1

5'- AACAGTGAAGTCGCGCTGGA-3'

5'- CAGCACAATGCCATAGGAGTAGACA-3'

145

NM012639

MKP

5'-ACAACCACAAGGCAGACATTAGCTC-3'

5'- CAGATGGTGGCTGACCTGGA-3'

127

NM053769

JIP3

5'-AGCCTGCCTGCCAAGTACAAG-3'

5'-TCAGGTTGACACCAGCAGCAC-3'

148

NM001100673

HGK

5'-TTCACCATGTCATTCACCGAGA-3'

5'-CCAGCTGAGCGCTTACACCA-3'

99

NM001106904

HSP72

5'-GCTTTCACCTCAAGCCTTTGGA-3'

5'-CGGGCCTCATGCACACATAG-3'

117

NM212504

GAD D45

5'-AGGCAGCCAAGCTGCTCAA-3'

5'-ACGTCCCGGTCGTCATCTTC-3'

82

NM024127

GAD D153

5'-TGGAAGCCTGGTATGAGGATCTG-3'

5'-GAGGTGCTTGTGACCTCTGCTG-3'

175

NM024134

GAPDH

5'-GGCACAGTCAAGGCTGAGAATG-3'

5'-ATGGTGGTGAAGACGCCAGTA-3'

143

NM017008

Uchida et al. BMC Neuroscience 2010 11:84   doi:10.1186/1471-2202-11-84