Transcriptional activity of the mouse Dcx upstream regulatory sequences in embryonic stem cells during neuronal differentiation and in cerebellar cells. (a) Schematic representation of the mouse Dcx promoter constructs for reporter gene expression. Cells were co-transfected with Dcx promoter constructs or the basic vector (promoterless luciferase vector; CTL) and control pSV-β-Galactosidase vector. Luciferase activity (expressed in RLU), of each transfection was determined after 48 hours and normalized to the corresponding internal control, β-Galactosidase activity (OD at 420 nm). (b) ESR1 cells were transfected at DD6 program and (c) CGN were extracted from PND3 mice and transfected the same day. Each value represents the mean ± SEM of at least three independent transfection experiments, each performed in triplicate. Asterisks mean significantly different from pdcx2kb values at P < 0.05 (*) or P < 0.01 (**).
Piens et al. BMC Neuroscience 2010 11:64 doi:10.1186/1471-2202-11-64