Table 1

Sequence of PCR cloning primers

Construct

Forward primer1

Reverse Primer 1


pMayven-EGFP

(BglII)5'-AGATCTCCCACCATGGAGACGCCGCCGCTGCCT-3'

(HindIII) 5'-AAGCTTTAATGGTTTATCAATAACTGT-3'


pΔSH3

(BglII) 5'-AGATCTCCCACCATGTGCACAAAGCAGGGTCATCAG-3'

(HindIII) 5'-AAGCTTTAATGGTTTATCAATAACTGT-3'


pΔFYN

(BglII) 5'-AGATCTCCCACCATGAACGAACTAAGAAGTCAA-3'

(HindIII) 5'-AAGCTTTAATGGTTTATCAATAACTGT-3'


pΔBTB/POZ

(BglII) 5'-AGATCTCCCACCATGGTCCTCCTCCCAGCAGCTGGC-3'

(HindIII) 5'-AAGCTTTAATGGTTTATCAATAACTGT-3'


pΔBACK

(BglII) 5'-AGATCTCCCACCATGAACCTTCCCAAATTGATG-3'

(HindIII) 5'-AAGCTTTAATGGTTTATCAATAACTGT-3'


pΔKELCH

(BglII)5'-AGATCTCCCACCATGGAGACGCCGCCGCTGCCT-3'

(HindIII) 5'-AAGCCTTTTGGGAAGGTTCATGGGTGT-3'


pΔKELCH-456

(BglII)5'-AGATCTCCCACCATGGAGACGCCGCCGCTGCCT-3'

(HindIII) 5'-AAGCCTACCTCCAACAACGCCAACGCC-3'


pΔKELCH-6

(BglII)5'-AGATCTCCCACCATGGAGACGCCGCCGCTGCCT-3'

(HindIII) 5'-AAGCCTACCATTAACTGCGCAGACACC-3'


pΔPPL

(HindIII) 5'-AGATCTCCCACCATGCCTCCCGCATGCACAAAGCAG-3'

(HindIII) 5'-AAGCTTTAATGGTTTATCAATAACTGT-3'


pQA

(HindIII) 5'-AGATCTCCCACC ATGGAGACGCAGCCGCTGCCTGCCGCA-3'

(HindIII) 5'-AAGCTTTAATGGTTTATCAATAACTGT-3'


pDK

(HindIII)5'-AGATCTCCCACCATGGACAAGCCGCCGCTGCCTCCCGCA-3'

(HindIII) 5'-AAGCTTTAATGGTTTATCAATAACTGT-3'


pEGFP-Mayven

(XhoI) 5'-CTCGAGATGGAGACGCCGCCGCTGCCT-3'.

(HindIII) 5'-AAGCTTTAATGGTTTATCAATAACTGT-3'


pEGFP-ΔFYN

(XhoI) 5'-CTCGAG-ATGAACGAACTAAGAAGTCAA-3'.

(HindIII) 5'-AAGCTTTAATGGTTTATCAATAACTGT-3'


pMayven-HA

(HindIII) 5'-AGCTTCCCACCATGGAGACGCCGCCGCTGCCT-3'

(Kpn I) 5'-GGTACCTAATGGTTTATCAATAACTGT-3'.


1 Restriction enzyme sites are italicised and corresponding sequences are in bold. The artificial Kozak motif is underlined.

Common primers used in the construction of the three sets of Mayven mutants.

Montague et al. BMC Neuroscience 2010 11:63   doi:10.1186/1471-2202-11-63

Open Data