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Open Access Research article

Subcellular localization of Mayven following expression of wild type and mutant EGFP tagged cDNAs

Paul Montague1, Peter GE Kennedy2 and Susan C Barnett1*

Author Affiliations

1 Division of Clinical Neuroscience, Glasgow Biomedical Research Centre, Room 4B17, 120 University Place, University of Glasgow, Glasgow G12 8TA, UK

2 Division of Clinical Neurosciences, Southern General Hospital, University of Glasgow, Glasgow G54 5TF, UK

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BMC Neuroscience 2010, 11:63  doi:10.1186/1471-2202-11-63

Published: 26 May 2010

Additional files

Additional file 1:

Time lapse recording of pEGFP O2A-cmyc cells. Transfectants were visualised over 18 hr period and images collected every three min using a Nikon TE2000 with perfect focus. It can be seen that the entire cell is filled with fluorescence.

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Open Data

Additional file 2:

Time lapse recording of pMayven-EGFP O2A-cmyc cells. Transfectants were visualised over 18 hr period and images collected every three min using a Nikon TE2000 with perfect focus. EGFP Mayven can be seen to move along the tips of the cells.

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Open Data

Additional file 3:

Time lapse recording of a SH3 mutant Mayven-EGFP in O2A-cmyc cells. Transfectants were visualised over 18 hr period and images collected every three min using a Nikon TE2000 with perfect focus. In this mutant Mayven remain perinuclear and can not be seen to be transported in the cell processes.

Format: AVI Size: 16.3MB Download file

Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more

Open Data