Figure 6.

BV-2 microglia and HEK293-GPR18 transfected, but not HEK293 wildtype, cells express GPR18. Immunofluorescent confocal microscopy was conducted using an antibody against the GPR18 C-terminus (1:150; green), phalloidin to label actin (1:40; red), and DAPI (1.5 μg/ml) to label the nucleus (blue). a, HEK293 wildtype with DAPI and phalloidin. b, HEK293 wildtype with GPR18 antibody and phalloidin. c, HEK293 wildtype with GPR18 antibody, DAPI, and phalloidin. d, HEK293-GPR18 transfected with DAPI and phalloidin. e, HEK293-GPR18 transfected with GPR18 antibody and phalloidin. f, HEK293-GPR18 transfected with GPR18 antibody, DAPI, and phalloidin. g, BV-2 microglia with DAPI and phalloidin. h, BV-2 microglia with GPR18 antibody and phalloidin. i, BV-2 microglia with GPR18 antibody, DAPI, and phalloidin.

McHugh et al. BMC Neuroscience 2010 11:44   doi:10.1186/1471-2202-11-44
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