Autoradiography in the peri-infarction brain tissue and the corresponding areas in the non-ischemic tissue. Autoradiography shows the number of black silver particles in the peri-infarction brain tissue. The number of black silver particles increases from 2 to 18 h after administration with D-[1-3H(N)]-mannitol (E, F, G, H) in comparison with the corresponding areas in the non-ischemic tissue (A, B, C, D) being more marked at 12 and 18 h (G, H). The number of dark silver particles also increases in the non-ischemic brain tissue from 2 to 18 h (A, B, C, D), notably at 12 and 18 h (C, D), but the number of particles is evidently less than that in the ischemic brain tissue at corresponding time points. Figure 3I shows a significant increase in the black silver particles in the peri-infarction brain tissue at 6, 12 and 18 h following permanent MCAO. Scale bars: A-H, 20 μm.
Zeng et al. BMC Neuroscience 2010 11:153 doi:10.1186/1471-2202-11-153