Step-wise procedure and end result of the regression method. From the independent component representing the DMN, obtained with spatiotemporal group ICA (1), we used the subject-and-session specific timecourses (2) to reconstruct subject-specific 3D maps for the 10 subjects*2 sessions (3). As a verification, we calculated the group means of the sham and rTMS groups separately ('reconstructed IC4') and observed that the DMN-characteristic pattern of regional co-activations occurred in both groups, with an additional co-activation of the hippocampal and lateral temporal cortices after sham stimulation but not real stimulation (4). Upon formal testing, using groupwise within-subject component general linear modelling, the lateral temporal regions differed significantly between conditions, such that their activation was reduced after rTMS. The image is thresholded at z > 2.3, showing the extent of the reductions and subthreshold reductions in the bilateral hippocampus (5). The reverse contrast showed an increase in activation of the right caudate nucleus (not shown).
van der Werf et al. BMC Neuroscience 2010 11:145 doi:10.1186/1471-2202-11-145