Aggregation of beta-amyloid peptide after rotenone exposure. Photomicrographs of cultured hippocampal cells exposed to DMSO (A) or rotenone at concentrations of 1 nM (B), 10 nM (C) and 25 nM (D) for 48 hours, illustrating the profile of beta-amyloid immunoreactivity. Arrows indicate the aggregates (B). Rotenone at high concentrations promoted intense cell death demonstrated by the fragmented and retracted nuclei (C and D), there is no evidence of beta-amiloid aggregation. Nuclei were stained with hematoxylin. Scale bar = 50 μm.
Chaves et al. BMC Neuroscience 2010 11:144 doi:10.1186/1471-2202-11-144