Quantification of hyperphosphorylated tau after rotenone exposure. Illustrative images of the pattern of bands corresponding to hyperphosphorylated tau in hippocampal cells exposed to 0.5 nM of rotenone or DMSO for 48 hours (A). The 230 kDa bands are considered the insoluble fraction of hyperphosphorylated tau, which seems to be reduced after rotenone (B), the 130 kDa bands are considered as oligomers of hyperphosphorylated tau (C) and 52-kDa bands as monomers of hyperphosphorylated tau (D), which are increased after prolonged exposure to low concentration of rotenone. Normalization was performed by beta-actin (43 kDa) signal. Values are shown as percentage of control (DMSO) ± S.D. * p < 0.05 as compared to control (DMSO), according to Student's t-test. The experiment was repeated twice, which were run in triplicates.
Chaves et al. BMC Neuroscience 2010 11:144 doi:10.1186/1471-2202-11-144