Aggregation of alpha-synuclein after rotenone exposure. Photomicrographs illustrating the immunoreactivity of alpha-synuclein in cultured cells of the locus coeruleus exposed to DMSO (A) or 0.5 nM rotenone (B) and hippocampal cells exposed to 1 nM of rotenone (C) for 48 hours. Arrows indicate cells with dense cytoplasm aggregates of alpha-synuclein. Arrowheads indicate sites of alpha-synuclein aggregation (detail in B). Cells were counterstained with hematoxylin (purple) after immunoreaction. Scale bar = 50 μm.
Chaves et al. BMC Neuroscience 2010 11:144 doi:10.1186/1471-2202-11-144