Analysis of rotenone toxicity. Photomicrographs illustrating the appearance of hippocampal cells treated with 0.5 nM (A), 1 nM (B) and 10 nM (C) of rotenone after addition of trypan blue in cell culture medium. Cells stained in blue (arrows) are under death process. Scale bar = 50 μm. Experiment was repeated three times. Cells from substantia nigra and locus coeruleus exhibited the same pattern of sensitivity.
Chaves et al. BMC Neuroscience 2010 11:144 doi:10.1186/1471-2202-11-144