Additional file 2.
Movement of the axonal framework occurs during normal axonal elongation of adult sensory neurons. Sensory neurons from adult rats were grown, labeled, and observed using the same conditions as the neurons from neonatal animals. The time of image acquisition for the phase and fluorescent images is shown as h:min in the movie. The movies were straightened to follow the right axonal branch using the Straighten plugin in ImageJ. A kymograph illustrating the forward advance of mitochondria, stably docked to the axonal framework, is shown on the right hand side of the movie. A kymograph illustrating the forward advance of mitochondria, stably docked to the axonal framework, is shown on the right hand side of the movie. The kymograph was generated from the straightened image. The length of the arrow above the kymograph represents 1 hour and the vertical scale bar is equal to 20 µm. The dot that moves across the top of the kymograph denotes the time position in the movie. The movie is displayed at 13 frames per second.
Format: MOV Size: 1.5MB Download file
Lamoureux et al. BMC Neuroscience 2010 11:140 doi:10.1186/1471-2202-11-140