Confocal image analysis of BrdU, GFAP and p75NTR co-immunostaining. Coronal sections of the dentate gyrus derived from a p75 NTR (+/-) mouse ware examined. Tissue was obtained one day following the 6 day BrdU injection protocol. (A) BrdU antibody (red) detects cells primarily located in the SGZ and hilus. (B) GFAP staining (purple) demonstrates a characteristic fibrillar pattern of astrocytes in the dentate gyrus. (C) p75 NTR staining (green) identifies cells in the SGZ. (D) Overlay of images shown in A-C reveals cells co-labeled with BrdU, GFAP and p75 NTR (white, horizontal arrow); BrdU and p75 NTR (yellow, down-up arrow); BrdU and GFAP (pink, up-down arrow); GFAP and p75 NTR (cyan) and BrdU alone (red). Scale bar: 100 μm.
Bernabeu and Longo BMC Neuroscience 2010 11:136 doi:10.1186/1471-2202-11-136