Quantitation of Aβ Oligomer and Aggregated Aβ Levels. A shows a Western blot using Aβ oligomer specific antibody A11 on the left from each of two representative 6D11 treated and vehicle treated Tg mice. On the right, a Western blot using anti-Aβ monoclonal antibody 6E10 is shown. B shows a bar graph of the densitometric analysis of the major A11 immunoreactive band at ~55 kDa, in arbitrary O.D. units. C shows a bar graph of the levels of aggregated Aβ in 6D11 treated (n = 10) and vehicle treated Tg mice (n = 8) as determined by ELISA. There is no significant difference between the 6D11 treated and vehicle treated Tg mice in the levels of Aβ oligomers determined by Western blot or of aggregated Aβ determined by ELISA.
Chung et al. BMC Neuroscience 2010 11:130 doi:10.1186/1471-2202-11-130