SP-PLA-based detection of Aβ protofibrils in the presence of variable amounts of Aβ monomers. The PLA signals for detection of 45 pg/ml Aβ protofibrils were not noticeably affected by the presence of an up to 2.2 million-fold molar excess of Aβ monomers for the protofibril-specific mAb158-PLA (red squares), and up to 22,000-fold molar excess of Aβ monomers for the 82E1-PLA (blue diamonds). The red and the blue circles indicate the assay background for mAb158 and 82E1, respectively. Error bars indicate standard deviations from the mean for triplicates for each reaction.
Kamali-Moghaddam et al. BMC Neuroscience 2010 11:124 doi:10.1186/1471-2202-11-124