The generation of new neurons in the neurogenic regions of the olfactory bulb is impaired in R6/2 animals compared to controls. In the GCL the percentage of newly generated cells that differentiate into a neuronal phenotype is unchanged comparing WT and R6/2 animals, as determined by double-labeling for BrdU and NeuN (A, B). Calculation of absolute numbers of surviving new neurons in the GCL revealed significantly decreased neurogenesis in HD mice compared to controls (C). Analysis of differentiation in the GLOM demonstrated unchanged neuronal differentiation in this region as well. Calculation of the total number of newly generated neurons in the GLOM showed a reduction of more than 50% in R6/2 mice compared to WT controls (F). D and E show representative immunostainings for BrdU and NeuN in the GLOM. Error bars represent SD, * indicates p < 0.05. Scale bars represent 20 μm.
Kohl et al. BMC Neuroscience 2010 11:114 doi:10.1186/1471-2202-11-114