Expression pattern of mutant huntingtin in developing and mature neurons of the subventricular zone and the olfactory bulb in R6/2 mice. SVZ derived neurons originate from dividing GFAP expressing cells ("stem cells", B cells) that generate transit-amplifying cells (C cells) which in turn give rise to neuroblasts (A cells). DCX-expressing neuroblasts migrate along the RMS to the olfactory bulb and differentiate into different neuronal cell types in the GCL and the GLOM. Schematic representations of regions of adult neurogenesis in the SVZ/olfactory bulb (E, adapted from (35)). Aggregates of mutant huntingtin (green) are absent in GFAP expressing SVZ stem cells (red, A), EGF-R expressing transit-amplifying cells (red, B) and neuroblasts with DCX immunoreactivity (red, C), but appear in DARPP-32 positive mature neurons of the adjacent striatum (red, D) of R6/2 mice. Moreover, neuroblasts of the RMS (F), the GCL (G) and the GLOM (H) do not show immunoreactivity for mutant huntingtin. In contrast, aggregates of mutant huntingtin (green) were detected in mature, calretinin-expressing neurons (red) of the GCL (I) and the GLOM (J) and in TH expressing neurons of the GLOM (red, K) of the olfactory bulb. Topro-3 (blue) was used as counterstain for cell nuclei. Scale bar represents 20 μm.
Kohl et al. BMC Neuroscience 2010 11:114 doi:10.1186/1471-2202-11-114