Phenotype of nSFEB-, SNP- and MNP-derived neural cell populations. Schematic illustration of the generation of nSFEBs and MNPs from ES cells (a). Low magnification overview (b; d) and regions of interests (ROIs) presented at higher magnifications (c; e; f) with nestin+, GFAP+ or βtubulin+ cells in adherent nSFEB (b; c), SNP (d; e) or MNP (f) cultures at different time points indicated by dap (days after plating) are given. Adherently growing nSFEB aggregates are delineated by a white line (b). White boxes indicate ROIs in b and d which were presented at higher magnification in e and c (brackets indicate the corresponding ROI). In c, iv + v and e, i + ii ROIs are illustrating the same region at different z-levels (the z-level given in μm), respectively.
Illes et al. BMC Neuroscience 2009 10:93 doi:10.1186/1471-2202-10-93