Additional file 1.

Schematic representation of method and spectra. Schematic representation of the (A) CCD-imaging setup used for calcium imaging and (B) simultaneous measurements of [Ca2+]i and [Ca2+]mito in SH-SY5Y cell line preparations. C) Spectral view of calcium imaging. The fluorescence excitation at 510 nm and emission detected at 340 nm are shown for Ca2+-saturated (A) and Ca2+-free (B) Fura-2 in pH 7.2 buffer. D) Spectral view of simultaneous [Ca2+]i and [Ca2+]mito measurements generated by the Molecular Probes spectral view program. Fluorescence excitation of fura-2 was done at 390 nm and 550 nm of rhod-2, respectively; emission was at 510 nm (fura-2, solid line) and at 600 nm (rhod-2) separated by a 565 nm dichroic mirror.

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Jaiswal et al. BMC Neuroscience 2009 10:64   doi:10.1186/1471-2202-10-64