Identification and quantification of different cell types targeted by injection of CMV-EGFP in the DG. Examples of EGFP co-localization with different markers of neuronal differentiation within the GCL. The orthogonal projections onto the x-z (bottom) and y-z (right) planes of cells indicated by hairlines are shown to confirm double labeling throughout the extent of EGFP+ cells co-expressing DCX and NeuN (A); Ki67 (B); GFAP (C); NeuN (D) or Nestin and NeuN (E). Each image shown is representative of 5 animals independently injected. Scale bars: 20 μm. F: Percentual distribution of EGFP+ cells expressing differentiation markers within the GCL, 1 week after the stereotaxic injection normalized to the total number of EGFP+ cells.
van Hooijdonk et al. BMC Neuroscience 2009 10:2 doi:10.1186/1471-2202-10-2