Confocal and epifluorescence images of MMP-7 immuno-reactivity in the CNS of wt mice during EAE. Brain sections were taken from a wt mouse 15 days after vaccination with MOG, when the EAE clinical score was 1. (a) Fluorescence microscope image of MMP-7 immunostaining in the brain of a wt mouse, scale bar = 50 μm. (b) Confocal microscope image of MMP-7 immunostaining in another region, scale bar = 10 μm. (c) MMP-7 immunostaining (red) shows strong labelling of many cells in the vascular cuff, (d) which co-localizes with Iba-1 immunoreactivity (green). (e) DAPI staining reveals the nuclear morphology of all cells in the cuff and surrounding parenchyma. (f) A merged image of c-e shows co-localization of Iba-1 and MMP-7 in the same cells. Arrows indicate double-stained cells in the parenchyma that have a spread-distended appearance. The lumen of the blood vessel is indicated with "v", scale bar = 100 μm.
Buhler et al. BMC Neuroscience 2009 10:17 doi:10.1186/1471-2202-10-17