Wild-type and mmp7-/- mice show respond differently to vaccination with MOG35–55. (a) Mean clinical EAE scores from wt (n = 12) and mmp7-/- (n = 9) mice injected with MOG35–55: graphed values are from two separate experiments. The first cadre of mice were sacrificed at day 15 for histological analysis (blue circles). mmp7-/- mice did not show clinical signs of EAE. Error bars represent SEM (*p < 0.05; **p < 0.005). (b) Haematoxylin and eosin staining of a brain section from a MOG-primed WT mouse shows perivascular cuffing of mononuclear cells. (c) Haematoxylin and eosin staining of a comparable section from a MOG-primed mmp7-/- mouse shows no vascular cuffing. (d) Spinal cord sections from MOG-primed wt mouse showing cuffing (e) that was not seen in spinal cord sections from MOG-primed mmp7-/- mice. (f) Spinal cord section from a MOG-primed wt mouse showing immune cells infiltration at the lateral edge and deeper into the parenchyma, (g) which was not seen in any spinal cord sections from similarly treated mmp7-/- mice. Scale bars = 100 μm.
Buhler et al. BMC Neuroscience 2009 10:17 doi:10.1186/1471-2202-10-17