The effect of spinalization and locomotor training on the intensity of synaptophysin immunofluorescence in the surrounding of perikarya of large neurons of lamina IX, in L3/L4 segments of the spinal cord. Optical density of synaptophysin immunofluorescence (IF) around perikarya was measured as indicated in Figure 2B and expressed in gray scale levels as the group means (± SD). Three sections per rat from L3/L4 segments were chosen for analysis. The mean numbers of large neurons per rat, selected for further analysis, were 28, 33 and 25 in the intact (n = 6), spinal (n = 6), and spinal trained (n = 5) rats, respectively. Two and three asterisks correspond to p < 0.01 and p < 0.001, respectively (Tukey-Kramer post-hoc test).
Macias et al. BMC Neuroscience 2009 10:144 doi:10.1186/1471-2202-10-144