Figure 7.

pEGFP-N1-hPGRN cloning construct. The human PGRN cDNA (hpgrn) was cloned into the pEGFP-N1 plasmid using EcoR1 and Sal1 restriction enzyme digestion sites. The eGFP molecule is fused to the C-terminus of the PGRN protein, and therefore does not affect its N-terminal signal sequence that is required for entry into the endoplasmic reticulum. Individual 12 cysteine granulin modules are designated 1 through to 7 and A through to G. P is paragranulin, a half-granulin module bearing six cysteine residues.

Ryan et al. BMC Neuroscience 2009 10:130   doi:10.1186/1471-2202-10-130
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