Short hairpin RNA silencing of PGRN in NSC 34. (A) RT-PCR of PGRN and actin in NSC34 cells stably -transfected with shRNA. (B) Relative expression ratio of quantitative RT-PCR showing the expression of PGRN mRNA in NSC34/pRS plasmid and NSC34/shPGRN. (C) Western blot analysis of PGRN and actin in NSC34 cells with stably-transfected with shRNA.
Ryan et al. BMC Neuroscience 2009 10:130 doi:10.1186/1471-2202-10-130