Figure 4.

Apoptotic cells were found in discrete clumps in areas of neurogenesis in the floating spheres. Apoptotic cells (A, TUNEL in green) were expressed in cells adjacent to neural progenitor markers in the floating spheres (B, β-III tubulin in red) indicating that spheres that have been treated to induce formation of neural progenitors contain areas of apoptosis. DAPI is shown in blue. Scale bar is 50 microns.