Characterization of human neural stem cells (NSCs) transduced with Olig2 transcription factor. (A, B) Phase contrast images of the parental NSCs (F3) and F3.Olig2 NSCs. (C) Comparison of gene expression by RT-PCR analysis between F3 and F3.Olig2 NSCs. (D, E) Confirmation of Olig2 transcription factor expression in F3.Olig2 cells (E). Olig2 expresssion was not observed in F3 cells (D). (F-K) In vitro immunocytochemical detection of oligodendrocyte lineage markers as indicated. Cells were grown on coverslips in DMEM with 2% FBS for 5 days and then fixed with 4% paraformaldehyde. (F, H, J) F3 cells, (G, I, K) F3.Olig2 cells. GalC = galactocerebrosidase, MBP = myelin basic protein. Scale bar = 50 μm.
Hwang et al. BMC Neuroscience 2009 10:117 doi:10.1186/1471-2202-10-117