Figure 7.

Effects of celastrol treatment on the climbing activity of DJ-1A RNAi flies and oxidative stress sensitivity of DJ-1A genomic deletion flies. A: Ddc-Gal4>UAS-DJ-1A RNAi flies, Ddc-Gal4>UAS-DJ-1A RNAi + hDJ-1 flies, and control wild type flies were treated with 20 μg/ml celastrol or mock-treated for 20 days before they were subjected to the climbing activity assay, in which the flies were bumped to the bottom of a plastic vial and the percentage of flies climbing to the top half of the vial after 7 seconds was quantified. Asterisks indicate P < 0.05 in Student's t-test. B: DJ-1A genomic deletion flies (Δ72) and control wild type (w-) flies were treated with 20 μg/ml celastrol or mock-treated for 20 days before they were subjected to the oxidative stress assay, in which the flies were exposed to 0.5% H2O2 and the survival rate over a 100 hour period was quantified. Asterisks indicate P < 0.05 in Student's t-test.

Faust et al. BMC Neuroscience 2009 10:109   doi:10.1186/1471-2202-10-109
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