Figure 1.

Whole-mount confocal immunofluorescence analysis of TH+ neurons in the DMC of Ddc-Gal4>DJ-1A RNAi flies. Whole-mount brain samples of 25-day old Ddc-Gal4>UAS-DJ-1A RNAi (A) or control Ddc-Gal4>UAS-GFP (B) flies were immunostained with anti-TH antibody and TH-positive immunofluorescence signals were detected by confocal microscopy. Representative confocal images of the TH+ neurons in the DMC are shown. Asterisks mark the identifiable TH+ neurons. Note that not only was there a reduction of TH+ neurons in Ddc-Gal4>UAS-DJ-1A RNAi flies compared to the control, the intensity of TH immunoreactivity was also reduced in the remaining neurons.

Faust et al. BMC Neuroscience 2009 10:109   doi:10.1186/1471-2202-10-109
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