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Open Access Highly Accessed Research article

Labelling and targeted ablation of specific bipolar cell types in the zebrafish retina

Xiao-Feng Zhao12, Staale Ellingsen13 and Anders Fjose1*

Author Affiliations

1 Department of Molecular Biology, University of Bergen, PO Box 7803, N-5020 Bergen, Norway

2 Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, MI 48109, USA

3 NIFES, National Institute of Nutrition and Seafood Research Strandgaten 229, N-5004 Bergen, Norway

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BMC Neuroscience 2009, 10:107  doi:10.1186/1471-2202-10-107

Published: 27 August 2009

Additional files

Additional file 1:

Figure S1. Transactivation of a UAS-regulated gene in retinal bipolar cells of the enhancer trap lines. Induced expression of red fluorescent protein (RFP) from a UAS:RFP construct in larvae at 3 dpf demonstrated for both transgenic lines the presence of Gal4-V16 in retinal bipolar cells, and its absence from the olfactory placodes in xfz43 (arrowheads). Scale bar: 50 μm.

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Additional file 2:

Figure S2. Retina recovery in larval progeny from the xfz3 × UAS:nfsB-mCherry mating. Retinal cryosections were obtained from larvae at different time points during a period of seven days following removal of Met at 5 dpf. The left panel (labelled 'no Met' at the top) show confocal images of retina cross-sections from untreated siblings expressing only Gal4-VP16/eGFP. The right panel (labelled 'Met 4–5 dpf') show confocal images of retina cross-sections from NTR-mCherry expressing larvae following removal of Met. The different time points and corresponding larval stages are: (C-E) 1 day post-treatment (6 dpf); (H-J) 3 days (8 dpf); (M-O) 5 days (10 dpf); (R-T) 7 days (12 dpf). The different types of fluorescence are indicated at the top. Scale bar: 50 μm.

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Additional file 3:

Figure S3. Retina recovery in larval progeny from the xfz43 × UAS:nfsB-mCherry mating. Confocal images of retina cross-sections from different stages of untreated siblings, which express only Gal4-VP16/eGFP (left panel), and Met treated larvae expressing NTR-mCherry (right panel) are arranged in the same way as described in Additional file 2: Figure S2. Scale bar: 50 μm.

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Additional file 4:

Figure S4. Apoptotic cells in retina of prodrug treated larvae from the xfz43 × UAS:nfsB-mCherry mating. (A, B) Higher magnifications are shown for areas from Figure 7L, O, respectively. Retina from Met treated larva (B) show rounded NTR-mCherry labelled cells lacking axons. The axon termini in both the IPL and OPL (arrowheads) are also disintegrating. Scale bar: 25 μm.

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Additional file 5:

Table S1. Genomic sequences flanking vector insertions. Zebrafish genomic sequences flanking the Tol2 vector insertions associated with retinal expression of Gal4-VP16/eGFP in the two transgenic lines. Tol2 vector sequences are underlined and coloured (right arm in red and left arm in blue). (Format: DOC).

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