DCs electroporated with CD40L RNA secrete IL-12. Panel A: Cytokine secretion profile of DCs transfected with 2 μg/million DC eGFP RNA and matured with the cytokine cocktail. Panel B: time course for the secretion of IL-10 versus IL-12 for DCs matured by transfection with 4 μg/million DC CD40L RNA and cultured in IFN-γ/PGE2. Panel C: IL-10 and IL-12 release as well as expression of the DC maturation markers, CD80 and CD83 from immature DC transfected with a range CD40L RNA concentrations, and immediately matured in the presence of IFN-γ.
Tcherepanova et al. BMC Molecular Biology 2008 9:90 doi:10.1186/1471-2199-9-90