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Resolution: standard / high Figure 1.
DCs electroporated with CD40L RNA secrete IL-12. Panel A: Cytokine secretion profile of DCs transfected with 2 μg/million DC eGFP
RNA and matured with the cytokine cocktail. Panel B: time course for the secretion
of IL-10 versus IL-12 for DCs matured by transfection with 4 μg/million DC CD40L RNA
and cultured in IFN-γ/PGE2. Panel C: IL-10 and IL-12 release as well as expression of the DC maturation markers,
CD80 and CD83 from immature DC transfected with a range CD40L RNA concentrations,
and immediately matured in the presence of IFN-γ.
Tcherepanova et al. BMC Molecular Biology 2008 9:90 doi:10.1186/1471-2199-9-90 |